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81.
Endocrine Disrupting Compounds Alter Risk‐Taking Behavior in Guppies (Poecilia reticulata) 下载免费PDF全文
Melissa M. Heintz Susanne M. Brander James W. White 《Ethology : formerly Zeitschrift fur Tierpsychologie》2015,121(5):480-491
Endocrine disrupting compounds (EDCs) enter aquatic habitats from a variety of anthropogenic sources and can mimic, block, or modulate the synthesis of natural hormones. EDCs affect both reproductive and non‐reproductive behaviors because hormones mediate responses associated with aggression and fear. We examined the effects of two EDCs on risk‐taking behaviors in guppies (Poecilia reticulata). We quantified risk‐taking in terms of propensity to forage in a risky location and tendency to join groups in the presence of a predator. We found that male and female guppies responded oppositely to environmentally relevant concentrations of an estrogenic EDC, 17α‐ethinylestradiol (EE2), or an androgenic EDC, 17β‐trenbolone (TB). Males decreased risk‐taking with increasing EE2 concentration (as predicted), but females increased risk‐taking (contrary to prediction). In contrast, females increased risk‐taking with increasing TB concentrations (as predicted), but males decreased risk‐taking (contrary to prediction). These results did not match our expectation that EE2 would reduce risk‐taking and TB would increase risk‐taking in both sexes. We suspect EE2 and TB produced these counterintuitive effects by downregulating their corresponding hormone receptors and thus reducing levels of circulating endogenous hormones in females and males, respectively. These results show that EDCs can alter fish behavior and potentially reduce fitness in unexpected ways. 相似文献
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Susanne Wegmann Eduardo A Maury Molly J Kirk Lubna Saqran Allyson Roe Sarah L DeVos Samantha Nicholls Zhanyun Fan Shuko Takeda Ozge Cagsal‐Getkin Christopher M William Tara L Spires‐Jones Rose Pitstick George A Carlson Amy M Pooler Bradley T Hyman 《The EMBO journal》2015,34(24):3028-3041
In Alzheimer's disease and tauopathies, tau protein aggregates into neurofibrillary tangles that progressively spread to synaptically connected brain regions. A prion‐like mechanism has been suggested: misfolded tau propagating through the brain seeds neurotoxic aggregation of soluble tau in recipient neurons. We use transgenic mice and viral tau expression to test the hypotheses that trans‐synaptic tau propagation, aggregation, and toxicity rely on the presence of endogenous soluble tau. Surprisingly, mice expressing human P301Ltau in the entorhinal cortex showed equivalent tau propagation and accumulation in recipient neurons even in the absence of endogenous tau. We then tested whether the lack of endogenous tau protects against misfolded tau aggregation and toxicity, a second prion model paradigm for tau, using P301Ltau‐overexpressing mice with severe tangle pathology and neurodegeneration. Crossed onto tau‐null background, these mice had similar tangle numbers but were protected against neurotoxicity. Therefore, misfolded tau can propagate across neural systems without requisite templated misfolding, but the absence of endogenous tau markedly blunts toxicity. These results show that tau does not strictly classify as a prion protein. 相似文献
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Susanne Pribbenow Marion L. East Andre Ganswindt Adrian S. W. Tordiffe Heribert Hofer Martin Dehnhard 《PloS one》2015,10(6)
Enzyme immunoassays (EIA) that measure faecal testosterone metabolites (fTM) are useful tools to monitor gonadal activity. The aim of this study was to validate an “in-house” epiandrosterone EIA to monitor fTM in spotted hyenas. FTM were characterised in a male and a female hyena that each received an injection of 3H-testosterone. High-performance liquid chromatography (HPLC) analyses revealed a cluster of highly polar enzyme-hydrolysable hormone metabolite conjugates. We performed hydrolysis using β-glucuronidase to deconjugate metabolites and improve sensitivity of the assay. Because β-glucuronidase from Helix pomatia has been reported to bias testosterone measurements in some species, we compared the enzymatic activity of the commonly used β-glucuronidase extracted from H. pomatia with the same enzyme from Escherichia coli. Our results showed that β-glucuronidases from both sources produced similar results from spotted hyena faeces. We therefore hydrolysed samples with H. pomatia enzymes. HPLC analyses also demonstrated that following hydrolysis the epiandrosterone EIA measured significant amounts of immunoreactive metabolites corresponding to radiolabelled metabolites in both sexes. Additionally, HPLC and GC-MS analyses confirmed the presence of epiandrosterone in faeces of spotted hyenas. The biological relevance of the epiandrosterone EIA was validated by demonstrating (1) a significant increase in fTM levels in response to a testosterone injection within 16 h, (2) no biological responsiveness to an adrenocorticotropic hormone (ACTH) injection and (3) significant differences in fTM levels between juvenile males and adult immigrant males in a free-ranging wild population. Our results clearly demonstrate that the epiandrosterone EIA is a reliable non-invasive method to monitor gonadal activity in spotted hyenas. 相似文献
85.
Cell and Tissue Research - The distribution, form variability and organization of two types of encapsulated mechanoreceptors, the Grandry and the Herbst corpuscles, were studied in the beak of... 相似文献
86.
Evelien Jongepier Susanne Foitzik 《Proceedings. Biological sciences / The Royal Society》2016,283(1822)
Division of labour is of fundamental importance for the success of societies, yet little is known about how individual specialization affects the fitness of the group as a whole. While specialized workers may be more efficient in the tasks they perform than generalists, they may also lack the flexibility to respond to rapid shifts in task needs. Such rigidity could impose fitness costs when societies face dynamic and unpredictable events, such as an attack by socially parasitic slavemakers. Here, we experimentally assess the colony-level fitness consequences of behavioural specialization in Temnothorax longispinosus ants that are attacked by the slavemaker ant T. americanus. We manipulated the social organization of 102 T. longispinosus colonies, based on the behavioural responses of all 3842 workers. We find that strict specialization is disadvantageous for a colony''s annual reproduction and growth during slave raids. These fitness costs may favour generalist strategies in dynamic environments, as we also demonstrate that societies exposed to slavemakers in the field show a lower degree of specialization than those originating from slavemaker-free populations. Our findings provide an explanation for the ubiquity of generalists and highlight their importance for the flexibility and functional robustness of entire societies. 相似文献
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Susanna Peters Jafargholi Imani Vera Mahler Kay Foetisch Susanne Kaul Kathrin E. Paulus Stephan Scheurer Stefan Vieths Karl-Heinz Kogel 《Transgenic research》2011,20(3):547-556
Pathogenesis-related protein-10 (PR10) is a ubiquitous small plant protein induced by microbial pathogens and abiotic stress
that adversely contributes to the allergenic potency of many fruits and vegetables, including carrot. In this plant, two highly
similar genes encoding PR10 isoforms have been isolated and designated as allergen Dau c 1.01 and Dau c 1.02. The aim of the
study was to generate PR10-reduced hypoallergenic carrots by silencing either one of these genes in transgenic carrots by
means of RNA interference (RNAi). The efficiency of gene silencing by stably expressed hairpin RNA (hnRNA) was documented
by means of quantitative RT-PCR (qPCR) and immunoblotting. Quantification of the residual protein revealed that PR10 accumulation
was strongly decreased compared with untransformed controls. Treatment of carrot plants with the PR protein-inducing chemical
salicylic acid resulted in an increase of PR10 isoforms only in wild-type but not in Dau c 1-silenced mutants. The decrease
of the allergenic potential in Dau c 1-silenced plants was sufficient to cause a reduced allergenic reactivity in patients
with carrot allergy, as determined with skin prick tests (SPT). However, simultaneous silencing of multiple allergens will
be required to design hypoallergenic carrots for the market. Our findings demonstrate the feasibility of creating low-allergenic
food by using RNAi. This constitutes a reasonable approach to allergen avoidance. 相似文献
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Thomas A. Davidson Helen Bennion Erik Jeppesen Gina H. Clarke Carl D. Sayer David Morley Bent V. Odgaard Peter Rasmussen Ruth Rawcliffe Jorge Salgado Gavin L. Simpson Susanne L. Amsinck 《Hydrobiologia》2011,676(1):299-315
Shallow lakes have been affected by a variety of human activities profoundly altering their ecological structure and function. Cladocerans have been used to track change resulting from a variety of drivers at a number of time scales. Aquatic macrophytes are well recognised as reflecting the ecological condition of a lake. Here, we compare the plant macrofossils with the sub-fossil cladoceran assemblages from 20 dated sediment cores. Co-correspondence analysis was used to determine the degree of commonality of change in community composition of the two biological groups through time. This analysis revealed very high levels of agreement in the nature and timing of change at all the sites examined with very high correlation coefficients between the axis 1 scores for macrofossils and cladocerans. Furthermore, at all sites a high proportion of the variance (min 20%, max 54%) in the macrofossil data was explained by the change in the cladoceran assemblage. Sub-fossil macrofossil and cladoceran assemblages, from at least from 1700 AD onwards, were examined in more detail at three sites: Ormesby Great Broad, Felbrigg Lake and Lake Søbygaard. There was very good accord in the main shifts of the cladoceran and macrofossil assemblages at all three sites. This may reflect the long-term shift in the principal focus of primary production from the benthic to the pelagic habitat. We suggest that the combination of their central position in the food-web and the presence of both pelagic and benthic taxa make cladocerans a strong candidate as the single best indicator of (palaeo) ecological condition related to changing trophic status and alteration in food-web structure in shallow lakes. 相似文献